111Indium-F(ab)'2-NCA 102 monoclonal antibody: in vitro study of a specific agent for the detection of inflammatory foci.
Identifieur interne : 004E08 ( Main/Exploration ); précédent : 004E07; suivant : 004E09111Indium-F(ab)'2-NCA 102 monoclonal antibody: in vitro study of a specific agent for the detection of inflammatory foci.
Auteurs : RBID : pubmed:8448572English descriptors
- KwdEn :
- MESH :
- chemical , diagnostic use : Antibodies, Monoclonal, Indium Radioisotopes.
- metabolism : Granulocytes.
- radionuclide imaging : Blood, Inflammation.
- ultrastructure : Bone Marrow, Liver.
- Animals, Humans, Mice, Mice, Inbred BALB C.
Abstract
A new antigranulocyte antibody was evaluated in vitro for the detection of inflammatory foci in man. The specificity for polymorphonuclear cells (PMN) of NCA 102, an anti-NCA 95 monoclonal IgG1, was determined with immunohistochemical and cytofluorometrical tests. Its affinity, assessed by Scatchard analysis, was 1.1 x 10(9) L/mol and the number of epitopes per granulocyte reached about 10(5). The biological properties of PMNs incubated with NCA 102 were not inhibited even when coupled with DTPA. A F(ab)'2 fragment was radiolabelled with 111Indium and incubated in the presence of whole blood. More than 65% radioactivity was selectively taken up by the PMN population. These findings indicated that NCA 102 antibody is suitable for sepsis detection.
PubMed: 8448572
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">111Indium-F(ab)'2-NCA 102 monoclonal antibody: in vitro study of a specific agent for the detection of inflammatory foci.</title><author><name sortKey="Collet, B" uniqKey="Collet B">B Collet</name><affiliation wicri:level="1"><nlm:affiliation>Centre Régional de Lutte contre le Cancer, Rennes, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre Régional de Lutte contre le Cancer, Rennes</wicri:regionArea><placeName><region type="région">Région Bretagne</region><settlement type="city">Rennes</settlement></placeName></affiliation></author><author><name sortKey="Maros, S" uniqKey="Maros S">S Maros</name></author><author><name sortKey="Moisan, A" uniqKey="Moisan A">A Moisan</name></author><author><name sortKey="Le Cloirec, J" uniqKey="Le Cloirec J">J Le Cloirec</name></author><author><name sortKey="Moinereau, M" uniqKey="Moinereau M">M Moinereau</name></author><author><name sortKey="Aumaitre, E" uniqKey="Aumaitre E">E Aumaitre</name></author><author><name sortKey="Toujas, L" uniqKey="Toujas L">L Toujas</name></author><author><name sortKey="Bourguet, P" uniqKey="Bourguet P">P Bourguet</name></author></titleStmt><publicationStmt><date when="1993">1993</date><idno type="RBID">pubmed:8448572</idno><idno type="pmid">8448572</idno><idno type="wicri:Area/Main/Corpus">005067</idno><idno type="wicri:Area/Main/Curation">005067</idno><idno type="wicri:Area/Main/Exploration">004E08</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (diagnostic use)</term><term>Blood (radionuclide imaging)</term><term>Bone Marrow (ultrastructure)</term><term>Granulocytes (metabolism)</term><term>Humans</term><term>Indium Radioisotopes (diagnostic use)</term><term>Inflammation (radionuclide imaging)</term><term>Liver (ultrastructure)</term><term>Mice</term><term>Mice, Inbred BALB C</term></keywords><keywords scheme="MESH" type="chemical" qualifier="diagnostic use" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Indium Radioisotopes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Granulocytes</term></keywords><keywords scheme="MESH" qualifier="radionuclide imaging" xml:lang="en"><term>Blood</term><term>Inflammation</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Bone Marrow</term><term>Liver</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Mice</term><term>Mice, Inbred BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A new antigranulocyte antibody was evaluated in vitro for the detection of inflammatory foci in man. The specificity for polymorphonuclear cells (PMN) of NCA 102, an anti-NCA 95 monoclonal IgG1, was determined with immunohistochemical and cytofluorometrical tests. Its affinity, assessed by Scatchard analysis, was 1.1 x 10(9) L/mol and the number of epitopes per granulocyte reached about 10(5). The biological properties of PMNs incubated with NCA 102 were not inhibited even when coupled with DTPA. A F(ab)'2 fragment was radiolabelled with 111Indium and incubated in the presence of whole blood. More than 65% radioactivity was selectively taken up by the PMN population. These findings indicated that NCA 102 antibody is suitable for sepsis detection.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">8448572</PMID><DateCreated><Year>1993</Year><Month>04</Month><Day>15</Day></DateCreated><DateCompleted><Year>1993</Year><Month>04</Month><Day>15</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0969-8051</ISSN><JournalIssue CitedMedium="Print"><Volume>20</Volume><Issue>2</Issue><PubDate><Year>1993</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Nuclear medicine and biology</Title><ISOAbbreviation>Nucl. Med. Biol.</ISOAbbreviation></Journal><ArticleTitle>111Indium-F(ab)'2-NCA 102 monoclonal antibody: in vitro study of a specific agent for the detection of inflammatory foci.</ArticleTitle><Pagination><MedlinePgn>175-82</MedlinePgn></Pagination><Abstract><AbstractText>A new antigranulocyte antibody was evaluated in vitro for the detection of inflammatory foci in man. The specificity for polymorphonuclear cells (PMN) of NCA 102, an anti-NCA 95 monoclonal IgG1, was determined with immunohistochemical and cytofluorometrical tests. Its affinity, assessed by Scatchard analysis, was 1.1 x 10(9) L/mol and the number of epitopes per granulocyte reached about 10(5). The biological properties of PMNs incubated with NCA 102 were not inhibited even when coupled with DTPA. A F(ab)'2 fragment was radiolabelled with 111Indium and incubated in the presence of whole blood. More than 65% radioactivity was selectively taken up by the PMN population. These findings indicated that NCA 102 antibody is suitable for sepsis detection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Collet</LastName><ForeName>B</ForeName><Initials>B</Initials><Affiliation>Centre Régional de Lutte contre le Cancer, Rennes, France.</Affiliation></Author><Author ValidYN="Y"><LastName>Maros</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Moisan</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Le Cloirec</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Moinereau</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Aumaitre</LastName><ForeName>E</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Toujas</LastName><ForeName>L</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Bourguet</LastName><ForeName>P</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>UNITED STATES</Country><MedlineTA>Nucl Med Biol</MedlineTA><NlmUniqueID>9304420</NlmUniqueID><ISSNLinking>0969-8051</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Indium Radioisotopes</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName><QualifierName MajorTopicYN="Y">diagnostic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Blood</DescriptorName><QualifierName MajorTopicYN="N">radionuclide imaging</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Bone Marrow</DescriptorName><QualifierName MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Granulocytes</DescriptorName><QualifierName MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Indium Radioisotopes</DescriptorName><QualifierName MajorTopicYN="Y">diagnostic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Inflammation</DescriptorName><QualifierName MajorTopicYN="Y">radionuclide imaging</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Liver</DescriptorName><QualifierName MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1993</Year><Month>2</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1993</Year><Month>2</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1993</Year><Month>2</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8448572</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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